The impact of urbanization on biodiversity has been well documented, yet research into the complex dynamics of ecological and evolutionary processes in urban areas is still in its infancy. When novel research challenges emerge, a horizon scan exercise is an integrated approach that brings together global interdisciplinary-minded individuals to identify future research questions that can influence new collaborations and funding agenda. Our horizon scan identified 30 questions for future research in urban evolutionary ecology covering themes in fundamental ecological and evolutionary processes, temporal and spatial scales, sustainability, climate change, sociopolitical and ethical considerations, and innovation in technology.
